The determination of iodine in food has always been difficult due to the low concentrations mgkg, difficult sample preparation and the volatility of iodine. Pdf determination of iodine value in triisocetyl citrate. This standard is issued under the fixed designation d 5768. Iodine value or number is the number of grams of iodine consumed by 100g of fat. Multiple regression equations were used to analyze the data to determine if mediation occurred between the variables. Test no determination method of analysis 12 determination of aflatoxin b1 and the sum of aflatoxin b1, b2, g1 and g2 in hazelnuts, peanuts, pistachios, figs, and paprika powder. The vegetable oil sample is reacted with an excess of iodine monochloride solution wijs reagent under. Iodine value was determined according to wijs method. Fresh palm oil had relatively lower iodine value of 45. Iodine value, also called iodine number or iodine index, is the mass of iodine in grams absorbed by 100 grams of oilfat. Fatty acids are long chain hydrocarbons with carboxyl groups cooh groups. Surplus methods are still available for historical and research purposes at the normal individual method rate. The results obtained when using carbon tetrachloride were compared to those obtained when using a substitute solvent mixture of cyclohexane and glacial acetic acid. The main objective of the study was to evaluate the.
This unsaturation is in the form of double bonds which react with iodine compounds. The time required for the measurement is thus reduced remarkably. Determination of acid value acidity, of iodine value, and of peroxide value corrigendaamendments to be made to methods published in the 7th edition new analytical methods developed and adopted by iupac commission vi. The nuclear regulatory commission had recommended that a method.
It may be determined by any of the following methods. The iodine value iv is a measure of total unsaturation of a lipid material. This standard is issued under the fixed designation d 5554. Full analyst members optional contractual analysis methods. Determination of iodine value of oil lipids laboratory. Determination of iodine value and acid value of red fruit oil. Dietary iodine intake in the etiology of cardiovascular disease stephen a. Introduction the iodine value of animal and vegetable fats and oils measures the amount of cc double bonds present in the product. It is expressed as the number of grams of iodine that will react with the double bonds in 100 grams of fats or oils the determination is conducted by dissolving a weighed sample in a nonpolar solvent such as cyclohexane, then adding glacial acetic. Preparation of polyvinyl alcoholxylan blending films with 1. The iodine value in chemistry is the mass of iodine in grams that is consumed by 100 grams of. Iv is a measure of the number of double bonds in the unsaturated fatty acids in one gram of oil.
To calculate the iodine number using the method described, below, all one need do is obtain the volume of na 2. A phase resetting curve prc measures the transient change in the phase of a neural oscillator subject to an external perturbation. Hanus solution iodine monobromide, freshly prepared starch solution, carbon tetrachloride,10% solution of potassium iodide, standard sodium thiosulphate solution n10. Normally, iodine is absorbed quite slowly but this method makes use of wijs, which is a stable solution consisting of iodine monochloride icl in acetic acid that reduces absorption time to approximately half an hour. The jocs standard methods for the analysis of fats, oils. Determination of iodine value and acid value of red fruit oil by infrared spectroscopy and multivariate calibration abstract a rapid method for the quantitative determination of iodine value iv and acid value av of red fruit oil rfo using ftir spectroscopy and multivariate calibration of partial least square pls is described. Although modern analytical methods yield more detailed and reliable information, structure indices are still widely used. Aocs surplus method cd 125 declared surplus 1993 wijs method for iodine value. Impact of frying on iodine value of vegetable oils before. The iodine value is a measure of the unsaturation of industrial oils and derivatives and is expressed in terms of the number of cg of iodine absorbed per g of test sample % iodine absorbed. Applicable to determination of iodine value for fats and oils which do not contain conjugated double bonds. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals.
The chemical reaction associated with this method of analysis involves. It purportedly serves a similar purpose in en 14214 as do the restrictions on methyl linolenate and fish oil esters. This quick wijs method gives almost equivalent value of hoffmans. Because the melting point and oxidative stability are related to the degree of unsaturation, iv provides an estimation of these quality factors. It is defined as the number of parts by mass of iodine absorbed by 100 parts by mass of an oil or fat. Total unsaturation of conjugated systems may be measured in accordance with test method d1541. A consistent definition of phase resetting using hilbert. Citations are the number of other articles citing this article, calculated by crossref and updated daily. Iodine value and method of analysis of iodine number for oil and fats.
Annex b describes a method for the calculation of the iv from fatty acid compositional data. Do not reference 8 182 glossary 183 absorbed dose, d 184 the absorbed dose is given by m d d d. This test method is essentially equivalent to test methods d 2078 and d 1959. Pdf standard test method for determination of iodine. Thanks to its simplicity, the innovative cdr method for the determination of iodine value iv in palm oil is ideal to be used during every process stage to monitor the quality of oil in real time and during the acceptance of crude oil, as well as during trading of finished products. Simple spectrophotometric method for determination of. Iodine value is a useful parameter in studying oxidative rancidity of oils since higher the unsaturation the greater the possibility of the oils to go rancid. This test method covers the determination of the iodine value of fatty amines, diamines, and amidoamines by the wijs procedure. In linoleic acid and cod liver oil the hanus method gives 2 to 4 per cent higher values than the other. To access content within the members area, you need to login by entering your username and password.
Stir the mixture until all the solids are fully incorporated. For example the theoretical iodine value of 100 g of linoliec acid. The wijs method as the standard for iodine absorption. This document specifies a reference method for the determination of the iodine value commonly known in the industry as iv of animal and vegetable fats and oils, hereinafter referred to as fats. Determination of the iodine value of oils and fats results.
The more the iodine number, the more the number of cc bonds. You can calculate theoretical iodine value based on number of unsaturation in a compound with known molecular mass and weight. Determination of iodine value iv in fats and oils with. This work demonstrates the ability of the elan icpms to measure iodine in food samples using a simple digestion procedure. This procedure, originally used for the analysis of oils and fats, is currently known as wijs. Determination of the iodine value of oils and fats. Trace and toxic metals in commonly consumed frozen fish in nigeria. Pipette out 10ml of fat sample dissolved in chloroform to an iodination flask labeled as test. Sources of errors in the determination of iodine value of palm oil by wijs method save as. A method for the determination of iodine value iv by fourier transformnear infrared ftnir spectroscopy was developed and evaluated in an international collaborative study. It was dissolved in a 250 ml erlenmeyer using 15 ml of chloroform and 25. Iodine value means the g value of halogen which adhere to g of methods such as hanus method and wijs method for iodine value.
Test method for measurement of iodine value of fats and oils stipulated in the below quoted standards is generally practiced by wijs method using monobasic iodine, however, hanus method is demonstrated here using iodine bromide. Iodine value of edible oils and fats according to wijs. When the catalyst is used, the reaction time of a sample with a wijs solution is as short as about 3 minutes. Add 20ml of iodine monochloride reagent in to the flask. Introduction fats and oils are a mixture of triglycerids. Addition of iodine chloride and backtitration with sodium thiosulphate by din 532411. Determination of iodine value in triisocetyl citrate citmol316 by united satates pharmacopeia hanus method article pdf available january 2019 with 1,324 reads how we measure reads. The international pharmacopoeia eighth edition pharmacopoea. These results could be attributed to the changes in the functionality properties of the blending films prepared in the different. Estimation of iodine value of an oil using hanus solution. The iodine value of an oilfat is the number of grams of iodine absorbed by. Pdf standard test method for determination of the iodine.
While phase is a fundamental concept, it has multiple definitions that may lead to contradictory results. The determination of iodine in food with the elan drce icpms. An annex describes a method for the calculation of the iv from fatty acid compositional data. Us4297106a rapid method for the measurement of iodine. Firstly, sample fat is dissolved in carbon tetrachloride, and hanus reagent is added and left in a dark room for. Formulations were prepared using the solid lipid, tween 80 as the hydrophilic surfactant, soya lecithin as the lipophilic surfactant. The processors of oil and fat are in need of a quick determination method of iodine value of oils and fats especially in case of checking the degree of. The i value iv of palm oil and fractions olein, stearin were determined by wijs method. The determination of the iodine values of fats and oils by.
Arrange all the reagent solutions prepared and the requirements on the table. The jocs standard methods for the analysis of fats, oils and related materials second english edition 20 japan oil chemists society. Sources of errors in the determination of iodine value of palm oil by wijs method. The iv, which indicates total unsaturation, has even been included in some standards for industrial products such as biodiesel. Various factors affecting results, and possible sources of error, were investigated. Estimation of iodine value of fats and oils theory. Hot homogenization method preparation of solid lipid nanoparticles via hot homogenization method was performed at a temperature above the melting point of lipid.
Estimation of iodine value of fats and oils procedure. A new calculation method for the determination of iodine value iv from measurements of fatty acid methyl esters is proposed. Determination of iodine value learn how to determine the iodine value using different methods iodine monochloride method or wijs method, iodine monobromide method or hanus method and pyridine bromide method. As a general rule, if the iodine number is in the range of 070, then youre looking at a fat. Determination of iodine species content in iodized salt. Two collaborative studies were conducted using the wijs method for determining the iodine value in a wide range of vegetable and animal oils and fats. Physicochemical properties of chemically and enzymatically interesterified palm oil and palm kernel oil blend fractions nur azimah kamarulzamana, norizzah abd. A green potentiometric method for the determination of the. Petroleum iodine value of oil and fat kyoto electronics. A higher iodine value indicates a higher degree of unsaturation. Teollisuuden voima oyj sisainen hovi petri measuring iodine from stack filters 20. Triglycerides are made up of three fatty acids linked to glycerol by fatty acyl esters.
Determination of iodine species content in iodized salt with various methods a. The iodine value is the number which expresses in grams the quantity of halogen, calculated as iodine, which is absorbed by 100 g of the substance under the described conditions. The iodine number is determinated with a carrier for the iodine, in which case the solution may be iodine monochloride in glacial acetic acid wijs. Hoption cann, phd department of health care and epidemiology, university of british columbia, vancouver, british columbia, canada. The official method for the determination of iodine 1 in milk has a lower limit of detection lld of 10 pcil 0.
All bsi british standards available online in electronic and print formats. Several variations of iodine value have been developed, although hanus method, wijs method and hubl method are still widely used. So it is important to know the extend to which a fatty acid is unsaturated. Scope applicable to normal industrial oils and derivatives, such as natural or synthetic drying. Oleaginous seeds the tests shown below are optional for all full analyst members in the oilseeds section. Method a iodine monochloride method or wijs method. The quantity of substance used in the determination should be such that at least 70% of the iodine added, as provided in the recommended procedure, is not absorbed. The prc encapsulates the dynamical response of a neural oscillator and, as a result, it is often used for predicting phaselocked modes in neural networks. These metrics are regularly updated to reflect usage leading up to the last few days. Iodine value iv the iodine value is a measure of the relative degree of unsaturation in oil components, as determined by the uptake of halogen. The method is based on the quantitative determination of fatty acid methyl esters of vegetable oils by capillary gas chromatography. Pdf a simple, fast, and green titrimetric method for the.
Standard test method for determination of iodine value of. Astm d2075 921998 standard test method for iodine value. Then to this mixture potassium iodide is added which reacts with remaining free. Surplus methods have been declared obsolete by the aocs uniform methods committee for one of the following reasons. In the measurement of iodine value by the wijs method, the measurement can be effected rapidly by using magnesium acetate or sodium acetate as a catalyst. The result is expressed as g of iodine i2 per 100 g of sample. On the determination of iodine value by the wijs method. Iodine value test with cdr palmoiltester palm oil analysis system. Dec 09, 20 ive carried out titrations to determine the iodine values of various oils and am using the equation bs x 0. The standard method en 14111 in the european biodiesel standard is based on the classic wet chemical method wijs for determining the iv. Determination of iodine value of fats and oil discover. Work in a well ventilated hood but avoid dispersing to. A critical study of the precision and value of halogen addition.
How do i calculate theoretical iodine value of an unsaturated. The wijs method as the standard for iodine absorption j. There are different methods for checking the unsaturation level in fatty acids, one among them is by determining the iodine value of fats. Precision data for the following methods published in the 7th edition. Simple methods via midir or 1h nmr spectroscopy for the. An aliquot of the sample was weighed according to the expected iodine number. The iodine value of a substance is the weight of halogens expressed as iodine absorbed by 100 parts by weight of the substance. The higher the iodine value, the more unsaturated fatty acid bonds are present in a fat. One of the most used procedures to determine the iodine value is known as wijs method. For the determination of the iodine number of oleic and ricin oleic acids both the hanus and the rosenmund methods give the same value which is very near the theoretical. The higher the iodine number is, the more unsaturated the fat. Members area iffo the marine ingredients organisation. Iodine production and industrial applications katja hora world iodine association wia leading manufacturers, formulators, and distributors of iodine recently established the world iodine association wia, whose role is to promote the use of iodine and provide relevant and accurate information about its applications and benefits. Determination of fat contents, iodine values, trace and.
Iodine value iv is a measure of the total number of double bonds present in fats and oils. The ftnir analyzer employed in this work uses disposable vials for sample handling and incorporates validation protocols designed to ensure that the calibration will give accurate results from analyzer to analyzer and. The misscare survey, nursing care reminders usage survey, and the impact of healthcare information technology scale were used to collect data to test for mediation. Standard methods for the analysis of oils, fats and. Rashida, norsyamimi misad a and zaliha omarb afaculty of applied sciences, universiti teknologi mara, 40450 shah alam, selangor, malaysia. Determination of iodine value of oil the iodine value is a measure of the degree of unsaturation in an oil. Alternative solvent system for iodine value determination. The iodine value of oil, which measures the degree of unsaturation of the oil, is usually determined by wijs method. Dry method the simulated iodized salt sample was made by mixing potassium iodate 122.
Use of mercuric acetate permits reduced reaction time compared to test methods d 2078 30 min and d 1959 1 h. Iodine value sample substance chemicals titrant instruments results chemistry accessories indication dm140sc at sensor 2 iodine value of edible oils and fats according to wijs aoac method 1 dissolve the sample in ccl 4, add 25 ml wijs solution and keep in the dark for approx. A simple, fast, and green titrimetric method for the determination of the iodine value of vegetable oils without wijs solution icl article pdf available in food analytical methods 99. Hanus method, animal and vegetable fats and oilsdetermination of iodine value, aoac 920. Mediation was tested using the method described by baron and kenny. Iodine value is a measure of the degree of unsaturation of an oil. Sources of errors in the determination of iodine value of. The wijs method, the wijs potentiometric method this paper, the visual method and the potentiometric method proposed in this paper were all applied. Impact of healthcare information technology on nursing. Simple spectrophotometric method for determination of iodine value of vegetable oils thidarat kruatian a and kritsana jitmaneea,b,c a department of chemistry and center of excellence for innovation in chemistry, faculty of science, chiang mai university, chiang mai 50200, thailand.