An aliquot of the sample was weighed according to the expected iodine number. The prc encapsulates the dynamical response of a neural oscillator and, as a result, it is often used for predicting phaselocked modes in neural networks. The more the iodine number, the more the number of cc bonds. Fresh palm oil had relatively lower iodine value of 45. The chemical reaction associated with this method of analysis involves. Iodine value sample substance chemicals titrant instruments results chemistry accessories indication dm140sc at sensor 2 iodine value of edible oils and fats according to wijs aoac method 1 dissolve the sample in ccl 4, add 25 ml wijs solution and keep in the dark for approx. Triglycerides are made up of three fatty acids linked to glycerol by fatty acyl esters. Various factors affecting results, and possible sources of error, were investigated.
Method a iodine monochloride method or wijs method. An annex describes a method for the calculation of the iv from fatty acid compositional data. Annex b describes a method for the calculation of the iv from fatty acid compositional data. Teollisuuden voima oyj sisainen hovi petri measuring iodine from stack filters 20. The time required for the measurement is thus reduced remarkably. Simple spectrophotometric method for determination of iodine value of vegetable oils thidarat kruatian a and kritsana jitmaneea,b,c a department of chemistry and center of excellence for innovation in chemistry, faculty of science, chiang mai university, chiang mai 50200, thailand. A higher iodine value indicates a higher degree of unsaturation. It is expressed as the number of grams of iodine that will react with the double bonds in 100 grams of fats or oils the determination is conducted by dissolving a weighed sample in a nonpolar solvent such as cyclohexane, then adding glacial acetic.
Formulations were prepared using the solid lipid, tween 80 as the hydrophilic surfactant, soya lecithin as the lipophilic surfactant. In the measurement of iodine value by the wijs method, the measurement can be effected rapidly by using magnesium acetate or sodium acetate as a catalyst. To calculate the iodine number using the method described, below, all one need do is obtain the volume of na 2. Introduction fats and oils are a mixture of triglycerids. Stir the mixture until all the solids are fully incorporated. Determination of iodine value of oil the iodine value is a measure of the degree of unsaturation in an oil.
Addition of iodine chloride and backtitration with sodium thiosulphate by din 532411. Estimation of iodine value of an oil using hanus solution. This unsaturation is in the form of double bonds which react with iodine compounds. Aocs surplus method cd 125 declared surplus 1993 wijs method for iodine value. A phase resetting curve prc measures the transient change in the phase of a neural oscillator subject to an external perturbation. Hanus method, animal and vegetable fats and oilsdetermination of iodine value, aoac 920.
Total unsaturation of conjugated systems may be measured in accordance with test method d1541. When the catalyst is used, the reaction time of a sample with a wijs solution is as short as about 3 minutes. While phase is a fundamental concept, it has multiple definitions that may lead to contradictory results. Determination of the iodine value of oils and fats results. It purportedly serves a similar purpose in en 14214 as do the restrictions on methyl linolenate and fish oil esters. The result is expressed as g of iodine i2 per 100 g of sample. Full analyst members optional contractual analysis methods. This standard is issued under the fixed designation d 5768.
The wijs method, the wijs potentiometric method this paper, the visual method and the potentiometric method proposed in this paper were all applied. The method is based on the quantitative determination of fatty acid methyl esters of vegetable oils by capillary gas chromatography. The determination of iodine in food has always been difficult due to the low concentrations mgkg, difficult sample preparation and the volatility of iodine. Introduction the iodine value of animal and vegetable fats and oils measures the amount of cc double bonds present in the product. Test method for measurement of iodine value of fats and oils stipulated in the below quoted standards is generally practiced by wijs method using monobasic iodine, however, hanus method is demonstrated here using iodine bromide. Estimation of iodine value of fats and oils theory. Determination of iodine value learn how to determine the iodine value using different methods iodine monochloride method or wijs method, iodine monobromide method or hanus method and pyridine bromide method. Pipette out 10ml of fat sample dissolved in chloroform to an iodination flask labeled as test. This document specifies a reference method for the determination of the iodine value commonly known in the industry as iv of animal and vegetable fats and oils, hereinafter referred to as fats. Iodine production and industrial applications katja hora world iodine association wia leading manufacturers, formulators, and distributors of iodine recently established the world iodine association wia, whose role is to promote the use of iodine and provide relevant and accurate information about its applications and benefits. Although modern analytical methods yield more detailed and reliable information, structure indices are still widely used. The determination of the iodine values of fats and oils by. Pdf standard test method for determination of iodine.
Determination of iodine value and acid value of red fruit oil. A green potentiometric method for the determination of the. It may be determined by any of the following methods. It is defined as the number of parts by mass of iodine absorbed by 100 parts by mass of an oil or fat. For example the theoretical iodine value of 100 g of linoliec acid. Two collaborative studies were conducted using the wijs method for determining the iodine value in a wide range of vegetable and animal oils and fats. Sources of errors in the determination of iodine value of palm oil by wijs method. Surplus methods are still available for historical and research purposes at the normal individual method rate. Iodine value iv the iodine value is a measure of the relative degree of unsaturation in oil components, as determined by the uptake of halogen.
Sources of errors in the determination of iodine value of. Iodine value means the g value of halogen which adhere to g of methods such as hanus method and wijs method for iodine value. Arrange all the reagent solutions prepared and the requirements on the table. This procedure, originally used for the analysis of oils and fats, is currently known as wijs. You can calculate theoretical iodine value based on number of unsaturation in a compound with known molecular mass and weight. Simple spectrophotometric method for determination of. Simple methods via midir or 1h nmr spectroscopy for the. Firstly, sample fat is dissolved in carbon tetrachloride, and hanus reagent is added and left in a dark room for. This work demonstrates the ability of the elan icpms to measure iodine in food samples using a simple digestion procedure.
Dry method the simulated iodized salt sample was made by mixing potassium iodate 122. Determination of iodine value iv in fats and oils with. Pdf determination of iodine value in triisocetyl citrate. Determination of iodine value of oil lipids laboratory. Estimation of iodine value of fats and oils procedure. Determination of iodine value and acid value of red fruit oil by infrared spectroscopy and multivariate calibration abstract a rapid method for the quantitative determination of iodine value iv and acid value av of red fruit oil rfo using ftir spectroscopy and multivariate calibration of partial least square pls is described. Pdf standard test method for determination of the iodine. This test method covers the determination of the iodine value of fatty amines, diamines, and amidoamines by the wijs procedure. The higher the iodine number is, the more unsaturated the fat. A critical study of the precision and value of halogen addition. Dietary iodine intake in the etiology of cardiovascular disease stephen a. A method for the determination of iodine value iv by fourier transformnear infrared ftnir spectroscopy was developed and evaluated in an international collaborative study.
Precision data for the following methods published in the 7th edition. To access content within the members area, you need to login by entering your username and password. Determination of iodine species content in iodized salt with various methods a. Applicable to determination of iodine value for fats and oils which do not contain conjugated double bonds. The determination of iodine in food with the elan drce icpms. Test no determination method of analysis 12 determination of aflatoxin b1 and the sum of aflatoxin b1, b2, g1 and g2 in hazelnuts, peanuts, pistachios, figs, and paprika powder. Iodine value or number is the number of grams of iodine consumed by 100g of fat. Citations are the number of other articles citing this article, calculated by crossref and updated daily. These metrics are regularly updated to reflect usage leading up to the last few days. Surplus methods have been declared obsolete by the aocs uniform methods committee for one of the following reasons. The vegetable oil sample is reacted with an excess of iodine monochloride solution wijs reagent under. The wijs method as the standard for iodine absorption.
The iodine value of oil, which measures the degree of unsaturation of the oil, is usually determined by wijs method. Sources of errors in the determination of iodine value of palm oil by wijs method save as. The iodine value iv is a measure of total unsaturation of a lipid material. The iodine value in chemistry is the mass of iodine in grams that is consumed by 100 grams of. The jocs standard methods for the analysis of fats, oils and related materials second english edition 20 japan oil chemists society. Standard methods for the analysis of oils, fats and. These results could be attributed to the changes in the functionality properties of the blending films prepared in the different. Thanks to its simplicity, the innovative cdr method for the determination of iodine value iv in palm oil is ideal to be used during every process stage to monitor the quality of oil in real time and during the acceptance of crude oil, as well as during trading of finished products. Because the melting point and oxidative stability are related to the degree of unsaturation, iv provides an estimation of these quality factors. Mediation was tested using the method described by baron and kenny. A consistent definition of phase resetting using hilbert.
The misscare survey, nursing care reminders usage survey, and the impact of healthcare information technology scale were used to collect data to test for mediation. This quick wijs method gives almost equivalent value of hoffmans. Do not reference 8 182 glossary 183 absorbed dose, d 184 the absorbed dose is given by m d d d. Fatty acids are long chain hydrocarbons with carboxyl groups cooh groups. All bsi british standards available online in electronic and print formats. Determination of the iodine value of oils and fats. The official method for the determination of iodine 1 in milk has a lower limit of detection lld of 10 pcil 0. Iodine value was determined according to wijs method. So it is important to know the extend to which a fatty acid is unsaturated. Hoption cann, phd department of health care and epidemiology, university of british columbia, vancouver, british columbia, canada. Trace and toxic metals in commonly consumed frozen fish in nigeria.
For the determination of the iodine number of oleic and ricin oleic acids both the hanus and the rosenmund methods give the same value which is very near the theoretical. The i value iv of palm oil and fractions olein, stearin were determined by wijs method. Standard test method for determination of iodine value of. It was dissolved in a 250 ml erlenmeyer using 15 ml of chloroform and 25. The iodine value is the number which expresses in grams the quantity of halogen, calculated as iodine, which is absorbed by 100 g of the substance under the described conditions. A simple, fast, and green titrimetric method for the determination of the iodine value of vegetable oils without wijs solution icl article pdf available in food analytical methods 99. This test method is essentially equivalent to test methods d 2078 and d 1959. Iodine value, also called iodine number or iodine index, is the mass of iodine in grams absorbed by 100 grams of oilfat. The iodine number is determinated with a carrier for the iodine, in which case the solution may be iodine monochloride in glacial acetic acid wijs. There are different methods for checking the unsaturation level in fatty acids, one among them is by determining the iodine value of fats. The processors of oil and fat are in need of a quick determination method of iodine value of oils and fats especially in case of checking the degree of. In linoleic acid and cod liver oil the hanus method gives 2 to 4 per cent higher values than the other. Normally, iodine is absorbed quite slowly but this method makes use of wijs, which is a stable solution consisting of iodine monochloride icl in acetic acid that reduces absorption time to approximately half an hour.
One of the most used procedures to determine the iodine value is known as wijs method. The iodine value is a measure of the unsaturation of industrial oils and derivatives and is expressed in terms of the number of cg of iodine absorbed per g of test sample % iodine absorbed. Iodine value of edible oils and fats according to wijs. Determination of iodine value of fats and oil discover. Several variations of iodine value have been developed, although hanus method, wijs method and hubl method are still widely used. Members area iffo the marine ingredients organisation. Oleaginous seeds the tests shown below are optional for all full analyst members in the oilseeds section.
Rashida, norsyamimi misad a and zaliha omarb afaculty of applied sciences, universiti teknologi mara, 40450 shah alam, selangor, malaysia. Determination of iodine species content in iodized salt. How do i calculate theoretical iodine value of an unsaturated. Alternative solvent system for iodine value determination. Iodine value iv is a measure of the total number of double bonds present in fats and oils. As a general rule, if the iodine number is in the range of 070, then youre looking at a fat.
Work in a well ventilated hood but avoid dispersing to. The international pharmacopoeia eighth edition pharmacopoea. A new calculation method for the determination of iodine value iv from measurements of fatty acid methyl esters is proposed. Physicochemical properties of chemically and enzymatically interesterified palm oil and palm kernel oil blend fractions nur azimah kamarulzamana, norizzah abd. The wijs method as the standard for iodine absorption j. The iv, which indicates total unsaturation, has even been included in some standards for industrial products such as biodiesel. Hanus solution iodine monobromide, freshly prepared starch solution, carbon tetrachloride,10% solution of potassium iodide, standard sodium thiosulphate solution n10. The iodine value of an oilfat is the number of grams of iodine absorbed by.
Preparation of polyvinyl alcoholxylan blending films with 1. Dec 09, 20 ive carried out titrations to determine the iodine values of various oils and am using the equation bs x 0. The standard method en 14111 in the european biodiesel standard is based on the classic wet chemical method wijs for determining the iv. Us4297106a rapid method for the measurement of iodine. The higher the iodine value, the more unsaturated fatty acid bonds are present in a fat. Impact of healthcare information technology on nursing.
This standard is issued under the fixed designation d 5554. Pdf a simple, fast, and green titrimetric method for the. Then to this mixture potassium iodide is added which reacts with remaining free. Add 20ml of iodine monochloride reagent in to the flask. Petroleum iodine value of oil and fat kyoto electronics. Iodine value is a measure of the degree of unsaturation of an oil. Hot homogenization method preparation of solid lipid nanoparticles via hot homogenization method was performed at a temperature above the melting point of lipid.
The jocs standard methods for the analysis of fats, oils. The nuclear regulatory commission had recommended that a method. Iodine value test with cdr palmoiltester palm oil analysis system. Scope applicable to normal industrial oils and derivatives, such as natural or synthetic drying. Iodine value and method of analysis of iodine number for oil and fats. The ftnir analyzer employed in this work uses disposable vials for sample handling and incorporates validation protocols designed to ensure that the calibration will give accurate results from analyzer to analyzer and. Iodine value is a useful parameter in studying oxidative rancidity of oils since higher the unsaturation the greater the possibility of the oils to go rancid. The main objective of the study was to evaluate the. Determination of acid value acidity, of iodine value, and of peroxide value corrigendaamendments to be made to methods published in the 7th edition new analytical methods developed and adopted by iupac commission vi. Astm d2075 921998 standard test method for iodine value. Determination of iodine value in triisocetyl citrate citmol316 by united satates pharmacopeia hanus method article pdf available january 2019 with 1,324 reads how we measure reads. Use of mercuric acetate permits reduced reaction time compared to test methods d 2078 30 min and d 1959 1 h.